Catalase activity in the red cell ghost of hypocatalasemia and normal subject. II. Isolation of catalase in the red cell ghost by electrophoresis.

For the purpose to clarify further the residual catalase in the blood cell ghost, the ghost has been applied to Cyanogum and starch block electrophoresis and the results are briefly summarized as follows. 1. It has been demonstrated that after Cyanogum electrophoresis of the ghost after several washings, bubbling due to enzymatic reaction of catalase occurs near the points of origin, when the plate is immersed in hydrogen peroxide solution and also it has been proved the presence of catalase so firmly bound to the ghost that it is hardly moved by the electro phoresis. Even with the ghost of hypocatlasemia there can be detected catalase which is likewise hardly eluted from the ghost. 2. In the estimation of catalase activities of each fraction from red cell ghost by starch block electrophoresis there can be detected catalase near the point of origin, that is not eluted by the electrophoresis, and the activity of which corresponds to about 0.1 % of the total red cell catalase activity. ∗PMID: 4246442 [PubMed indexed for MEDLINE] Copyright c ©OKAYAMA UNIVERSITY MEDICAL SCHOOL Acta Merl. Okayama 23, 553-558 (1969) CATALASE ACTIVITY IN THE RED CELL GHOST OF HYPOCATALASEMIA AND NORMAL SUBJECT II. ISOLATION OF CATALASE IN THE RED CELL GHOST BY ELECTROPHORESIS